Description: Ventilator-associated pneumonia (VAP) often stands as the leading cause of death related to hospital-acquired infections, especially due to the prevalence of multidrug-resistant (MDR) pathogens. Addressing this challenge requires innovative antimicrobial strategies. This study represents a comprehensive approach to explore the potential of antimicrobial peptides (AMPs) isolated from Lactobacillus acidophilus as an avant-garde therapeutic agent.
Endotracheal secretion samples were collected. Standard microbiological procedures were used to screen these samples for the presence and identification of pathogens. Following identification, the MDR pathogens were subjected to susceptibility testing against a panel of antimicrobial Drugs in order to determine their resistance patterns.
AMPs were extracted from isolated Lactobacillus strains and their characteristics were delineated using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The Cell-Free Supernatants (CFS) of Lactobacillus, enriched with AMPs, were then tested against the isolated MDR pathogens. Their efficacy was assessed via a Hemolysis Assay to evaluate toxicity levels.
Socio-Economic Benefits: Make sure the survival of neonates in ventilator
Category: Medicinal Products – Natural medicinal products
Department: Microbiology